Difference between revisions of "Part:BBa K364318:Design"

 
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<partinfo>BBa_K364318 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The reconstruction of the DBD to the hinge region, LBD and Backbone will be done with RFC 25  
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The construction of the LBD and pSB1C3 backbone have been done with RFC 25.
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===Source===
 
===Source===
  
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C. elegans orphan nuclear receptor  
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C. elegans orphan nuclear receptor. Artifically synthetized Ligand Binding Domain of C. elegans NHR-80 placed into pSB1C3 carrier vector.
  
 
===References===
 
===References===
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1. Miyabayashi T, Palfreyman MT, Sluder AE, Slack F, Sengupta P. "Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans." [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WDG-45GWBM7-2F-1&_cdi=6766&_user=2073021&_pii=S0012160699994708&_origin=search&_coverDate=11%2F15%2F1999&_sk=997849997&view=c&wchp=dGLzVzb-zSkzS&md5=cc520c758a508e80575a804387429b13&ie=/sdarticle.pdf Dev Biol. 1999 Nov 15;215(2):314-31.]

Latest revision as of 21:23, 25 October 2010

NHR-80 LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 232


Design Notes

The construction of the LBD and pSB1C3 backbone have been done with RFC 25.

Source

C. elegans orphan nuclear receptor. Artifically synthetized Ligand Binding Domain of C. elegans NHR-80 placed into pSB1C3 carrier vector.

References

1. Miyabayashi T, Palfreyman MT, Sluder AE, Slack F, Sengupta P. "Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans." [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WDG-45GWBM7-2F-1&_cdi=6766&_user=2073021&_pii=S0012160699994708&_origin=search&_coverDate=11%2F15%2F1999&_sk=997849997&view=c&wchp=dGLzVzb-zSkzS&md5=cc520c758a508e80575a804387429b13&ie=/sdarticle.pdf Dev Biol. 1999 Nov 15;215(2):314-31.]