Difference between revisions of "Part:BBa K364310:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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===Ligand===
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Ecdysteroids
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Ecdysteroids  are  insect  moulting  and  sex  hormones  which  include  ecdysone  and
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its  homologues  such  as  20-hydroxyecdysone.  Ecdysteroids  also  occur  in  other
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invertebrates where they can play a different role. They also appear in many plants
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mostly  as  a  protection  agents  (toxins  or  antifeedants)  against  herbivore  insects.
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These  phytoecdysteroids  have  medicinal  value  and  are  part  of  herbal  adaptogenic
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remedies like cordyceps.
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===Physiological effects of EcR===
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Pulses of 20-hydroxyecdysone occur during insect development, whereupon this hormone binds to the ecdysone receptor, a ligand-activated transcription factor found in the nuclei of insect cells. This in turn leads to the activation of many other genes, as evidenced by chromosomal puffing at over a hundred sites. Ultimately the activation cascade causes physiological changes that result in ecdysis (moulting).
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===Potential applications of EcR LBD===
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EcR LBD can be fused with different DBDs in order to activate downstream gene expression depending on the binding site of the DBD.
  
 
===Applications of BBa_K364310===
 
===Applications of BBa_K364310===

Latest revision as of 01:56, 26 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Ligand

Ecdysteroids

Ecdysteroids are insect moulting and sex hormones which include ecdysone and its homologues such as 20-hydroxyecdysone. Ecdysteroids also occur in other invertebrates where they can play a different role. They also appear in many plants mostly as a protection agents (toxins or antifeedants) against herbivore insects. These phytoecdysteroids have medicinal value and are part of herbal adaptogenic remedies like cordyceps.

Physiological effects of EcR

Pulses of 20-hydroxyecdysone occur during insect development, whereupon this hormone binds to the ecdysone receptor, a ligand-activated transcription factor found in the nuclei of insect cells. This in turn leads to the activation of many other genes, as evidenced by chromosomal puffing at over a hundred sites. Ultimately the activation cascade causes physiological changes that result in ecdysis (moulting).

Potential applications of EcR LBD

EcR LBD can be fused with different DBDs in order to activate downstream gene expression depending on the binding site of the DBD.

Applications of BBa_K364310

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