Difference between revisions of "Part:BBa K322211"
 
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<partinfo>BBa_K322211 short</partinfo>
 
<partinfo>BBa_K322211 short</partinfo>
  
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This is the same as BioBrick BBa-K322246 (muatnt S284T) but has lysine inserted between amino acids 355 and 356 of the native sequence. This has been reported to result in a red-shifted output.
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This is the same as BioBrick BBa-K322246 (muatnt S284T) but has lysine inserted between amino acids 355 and 356 of the native sequence. This results in a red shifted output (see Fujii et al, 2007).  
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Features: introduced lysine codon 1066 to 1068
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reference: Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136. Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:43, 7 October 2010

Red emitting firefly luciferase mutant (356K)

This is the same as BioBrick BBa-K322246 (muatnt S284T) but has lysine inserted between amino acids 355 and 356 of the native sequence. This results in a red shifted output (see Fujii et al, 2007).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808