Difference between revisions of "Part:BBa K322705"

Latest revision as of 15:41, 25 October 2010

E. coli TnaA (tryptophanase) upstream region (500bp)

This is part of a set of two biobricks (with TnaA downstream region -BBa_K322706-). It is to be used for targeting genes to the TnaA (Tryptophanase, which responsible for indole production in E. coli) with the BRIDGE recombineering method.

Any biobrick can be inserted between the upstream and downstream regions of TnaA, then inserted into the E. coli genome without leaving a marker using BRIDGE. Because it does not leave a marker, this method can be used repeatedly, and one could potentially replace the whole genome with biobricked versions.

This part is made of the 500 bases upstream of the TnaA start codon (and thus include RBS and promoter for whatever gene is inserted in its place).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K322705 short</partinfo>
-It is to be used for targeting genes to this locus in BRIDGE.
+This is part of a set of two biobricks (with TnaA downstream region -BBa_K322706-). It is to be used for targeting genes to the TnaA (Tryptophanase, which responsible for indole production in E. coli) with the BRIDGE recombineering method.
-In the BRIDGE, with this system, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with new genes.
+Any biobrick can be inserted between the upstream and downstream regions of TnaA, then inserted into the E. coli genome without leaving a marker using BRIDGE. Because it does not leave a marker, this method can be used repeatedly, and one could potentially replace the whole genome with biobricked versions.
+This part is made of the 500 bases upstream of the TnaA start codon (and thus include RBS and promoter for whatever gene is inserted in its place).