Difference between revisions of "Part:BBa K322237"

 
 
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<partinfo>BBa_K322237 short</partinfo>
 
<partinfo>BBa_K322237 short</partinfo>
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In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light
  
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In luminescent reactions, light is produced by the oxidation of a luciferin (a pigment):
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This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, present in BBa_I712019 (presumably to make a proper eukaryotic context for the start codon), were removed for improved expression in E. coli.
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luciferin + O2 &#8594; oxyluciferin + light
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Thus luciferase is a a reporter protein when connected to other proteins or promoters prodicing light, by the oxidation of substrate luciferin.  
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This is derived from BioBrick I712019, but PCR was used to remove the extra bases before the start codon (these are shown in the Registry sequencing data but are not indicated in the sequence as entered by Ljubljana 2007; presumably they are there to make a proper eukaryotic context for the start codon). This BioBrick contains the bare coding sequence in pSB1C3.
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Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:12, 7 October 2010

Firefly luciferase from Photinus pyralis In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light

This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, present in BBa_I712019 (presumably to make a proper eukaryotic context for the start codon), were removed for improved expression in E. coli.

Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808