Difference between revisions of "Part:BBa K322921:Design"

 
(Design Notes)
 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K322921 short</partinfo>
 
<partinfo>BBa_K322921 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
Note: MABEL was used to mutate an internal EcoRI site to GAGTTC (underlined).
+
MABEL was used to mutate an internal EcoRI site to GAGTTC.
Features: coding sequence runs from 13 to 1434, RBS 1 to 3, EcoRI site mutated 211 to 216.
+
 
+
 
+
 
+
  
 
===Source===
 
===Source===

Latest revision as of 16:07, 25 October 2010

B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

MABEL was used to mutate an internal EcoRI site to GAGTTC.

Source

Source: Bacillus subtilis 168 genomic DNA. Sequence (from SacBPlan11June10.docx)


References