Difference between revisions of "Part:BBa K310006:Design"

 
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<partinfo>BBa_K310006 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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I needed to perform site directed mutagenesis in the coding region of ArsR due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.  
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Site directed mutagenesis was performed in the coding region of ArsR due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.
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===Source===
 
===Source===
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===References===
 
===References===
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This paper was particularly helpful when working with the Ars Operon and Arsenic parts within E. coli: [http://aem.asm.org/cgi/reprint/70/8/4582 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR]

Latest revision as of 14:44, 27 October 2010

ArsR under the control of J23100, includes RBS B0034 and terminator B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199


Design Notes

Site directed mutagenesis was performed in the coding region of ArsR due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.

Source

I used PCR to isolate ArsR from E. coli K-12 MG1655 genome and attached B0015, B0034 and J23100 from the parts registry.

References

This paper was particularly helpful when working with the Ars Operon and Arsenic parts within E. coli: [http://aem.asm.org/cgi/reprint/70/8/4582 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR]