Difference between revisions of "Part:BBa K385003:Experience"
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===Applications of BBa_K385003===
 
===Applications of BBa_K385003===
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The Aberdeen 2010 iGEM team has no direct experience of using BBa_K385003, but the closely related part BBa_K385004. consisting of a tandem repeat of the N-peptide, allowed the functional expression of a downstream GFP reporter.
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The N-peptide reading frame was fused in-frame to GFP to make a translational fusion. It was placed under control of the yeast GAL1 promoter (BBa_J63006), and transformed into yeast in the single copy shuttle vector pRS415.
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The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed using a fluorescence microscope optimised for GFP visualisation (Figure 1).
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[[Image:N25 + Gal-3.jpg|center|400 px]]
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A control culture of the same transformant was grown using glucose as the carbon source;  these conditions do not activate the GAL promoter. The results (Figure 2) show no GFP fluorescence.
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Overall the results indicate that the N-peptide can be successfully expressed as a protein fusion with other standard parts.
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===User Reviews===
 
===User Reviews===

Latest revision as of 12:06, 27 September 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K385003

The Aberdeen 2010 iGEM team has no direct experience of using BBa_K385003, but the closely related part BBa_K385004. consisting of a tandem repeat of the N-peptide, allowed the functional expression of a downstream GFP reporter.

User Reviews

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