Difference between revisions of "Part:BBa K389013"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K389013 short</partinfo> | <partinfo>BBa_K389013 short</partinfo> | ||
− | With this system it is possible to measure the activity of a | + | With this system it is possible to measure the activity of a VirA receptor and the ''vir'' promoter, respectively. The reporter is the mRFP from the parts.igem. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This Device can be used for characterization and screening of mutated VirA receptors. | ||
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+ | For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find the complete working system here: <partinfo>K389016</partinfo> | ||
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Latest revision as of 17:05, 27 October 2010
VirA reporter system mRFP
With this system it is possible to measure the activity of a VirA receptor and the vir promoter, respectively. The reporter is the mRFP from the parts.igem.
Usage and Biology
This Device can be used for characterization and screening of mutated VirA receptors.
For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001. You can find the complete working system here: BBa_K389016
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1686
Illegal AgeI site found at 1798 - 1000COMPATIBLE WITH RFC[1000]