Difference between revisions of "Part:BBa K389012"

Latest revision as of 16:59, 27 October 2010

VirA reporter system luc

With this system it is possible to measure the activity of a VirA receptor and the vir promoter, respectively. The reporter is a firefly luciferase from Promega's pGL4.10[luc2] plasmid.

Usage and Biology

This Device can be used for characterization and screening of mutated VirA receptors.

For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001. You can find the complete working system here: BBa_K389015

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1195
Illegal NgoMIV site found at 2539
Illegal NgoMIV site found at 2560
Illegal AgeI site found at 2263
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2445

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K389012 short</partinfo>
-With this system it is possible to measure the activity of a ''virA'' receptor and the ''vir'' promoter, respectively. The reporter is a firefly luciferase from Promega's pGL4.10luc2 plasmid.
+With this system it is possible to measure the activity of a VirA receptor and the ''vir'' promoter, respectively. The reporter is a firefly luciferase from Promega's pGL4.10[luc2] plasmid.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This Device can be used for characterization and screening of mutated VirA receptors.
+For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find the complete working system here: <partinfo>K389015</partinfo>
 <!-- -->