Difference between revisions of "Part:BBa K404204"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404204 short</partinfo>
 
<partinfo>BBa_K404204 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404204 ViralBrick-453-Z34C)]
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|-
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|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_K404204 BBa_K404204]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Requirement'''
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|pSB1C3<br>
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|-
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|'''Source'''
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|
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|-
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|'''Submitted by'''
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|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
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|}
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<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
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<br><b>The Z34C antibody binding motif, ready for insertion into the 453 loop</b>
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[[Image:Freiburg10_ViralBrick-logo-453-Z34C.png|thumb|center|480px]]<br>
  
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x
 
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of  [http://www.ncbi.nlm.nih.gov/protein/153106 Staphylococcal Protein A]. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
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[[Image:Freiburg10_ViralBrick_motif_Z34C.png|thumb|center|480px]]
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===Restriction sites===
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<table border="1" style="float:right; width:480px; height:auto;>
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<tr>
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  <th><b>Restriction site</b></th>
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  <th><b>Enzyme</b></th>
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  <th><b>Recognition sequence</b></th>
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  <th><b>Activity</b></th>
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  <th><b>Temp.</b></th>
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</tr>
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<tr>
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  <td>upstream 453</td>
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  <td>[http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF]</td>
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  <td>[[image:Freiburg10_recognition site SspI-HF.Gif]]</td>
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  <td>25;100;0;100</td>
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<td>37°C</td>
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</tr>
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<tr>
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  <td>downstream 453</td>
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  <td>[http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF]</td>
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  <td>[[image:Freiburg10_recognition site SalI-HF.Gif]]</td>
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  <td>10;100;100;100</td>
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  <td>37°C</td>
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<tr>
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</table>
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 02:21, 28 October 2010

ViralBrick-453-Z34C

ViralBrick-453-Z34C)
BioBrick Nr. BBa_K404204
RFC standard RFC 25
Requirement pSB1C3
Source
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]















The Z34C antibody binding motif, ready for insertion into the 453 loop

Freiburg10 ViralBrick-logo-453-Z34C.png


Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of [http://www.ncbi.nlm.nih.gov/protein/153106 Staphylococcal Protein A]. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.

Freiburg10 ViralBrick motif Z34C.png


Restriction sites

Restriction site Enzyme Recognition sequence Activity Temp.
upstream 453 [http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF] Freiburg10 recognition site SspI-HF.Gif 25;100;0;100 37°C
downstream 453 [http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF] Freiburg10 recognition site SalI-HF.Gif 10;100;100;100 37°C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]