Difference between revisions of "Part:BBa K299510"
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Latest revision as of 23:42, 25 October 2010
Expression Vector pT7+J6100
This part is licensed under
Creative BioCommons
This is expression vector containing J61100 under T7 promoter. It can be used for easily expression of any protein-encoding BioBrick in RFC10 format.
Authors
Clonning:
- Ania Puławska
- Milena Bażlekowa
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Construct design
The device consists of T7 promoter followed by J61100 RBS. Device was created by annealing of synthetic complementary oligonucleotides followed by subsequent cloning into pSB1A2 vector.
Expression strength
Expression strength of this device is based on measurements of [http://2010.igem.org/Team:Warsaw/Stage1/PromMeas promoter strength] and [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS performance] done by Team Warsaw 2010.
It's estimated to be 4,29% relative to BBa_K299509, the strongest ExpressIt! vector.
Summary for the whole ExpressIt! family is shown below:
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 33
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 33
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 33
- 1000COMPATIBLE WITH RFC[1000]