Difference between revisions of "Part:BBa K302017"

 
 
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<partinfo>BBa_K302017 short</partinfo>
 
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Coding sequences and RBS' for spaIFEG cluster.
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Coding sequences and RBS' for ''spaIFEG'' cluster.
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Subtilin is a lantibiotic peptide produced naturally by ''Bacillus subtilis'' ATCC 6633 but not by ''Bacillus subtilis'' 168.
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''spaI'' encodes a membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores.
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''spaFEG'' encode three proteins which form a membrane transporter that removes subtilin from the membrane.
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Latest revision as of 15:15, 28 September 2010

spaIFEG cluster

Coding sequences and RBS' for spaIFEG cluster.

Subtilin is a lantibiotic peptide produced naturally by Bacillus subtilis ATCC 6633 but not by Bacillus subtilis 168.

spaI encodes a membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores.

spaFEG encode three proteins which form a membrane transporter that removes subtilin from the membrane.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 740
    Illegal BglII site found at 1488
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1035
    Illegal SapI site found at 1315
    Illegal SapI.rc site found at 2038