Difference between revisions of "Part:BBa J70627"

 
 
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__NOTOC__
 
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<partinfo>BBa_J70627 short</partinfo>
 
<partinfo>BBa_J70627 short</partinfo>
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This is a RFC12 (SpeI,NheI) compatible chitin binding domain.  It is an internal domain meant for either N or C terminal fusions.
 
This is a RFC12 (SpeI,NheI) compatible chitin binding domain.  It is an internal domain meant for either N or C terminal fusions.
  
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===Usage and Biology===
 
===Usage and Biology===
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Chitin binding domains are most commonly used with self-splicing intein tags such as <partinfo>J70628</partinfo>.  CBD tags are used primarily for protein purification due to the high yields and purity, and along with an intein domain the tag can be easily removed.  Note that the N or C terminal residue of the tagged protein can affect intein cleavage.  See <partinfo>J70629</partinfo> for the intein-CBD fusion.
  
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Common tag information:
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<ul>
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<li>Matrix: Chitin</li>
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<li>Elution: DTT or B-ME</li>
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<li>Yield/Purity: low milligram yields, 100% purity</li>
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<li># Steps: One step</li>
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<li>Fusion Location: Internal</li>
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<li>Affects Function?: Possibly, unlikely after intein cleavage</li>
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</ul>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 18:05, 11 August 2010

RFC12 Internal Chitin Binding Domain

This is a RFC12 (SpeI,NheI) compatible chitin binding domain. It is an internal domain meant for either N or C terminal fusions.

Usage and Biology

Chitin binding domains are most commonly used with self-splicing intein tags such as BBa_J70628. CBD tags are used primarily for protein purification due to the high yields and purity, and along with an intein domain the tag can be easily removed. Note that the N or C terminal residue of the tagged protein can affect intein cleavage. See BBa_J70629 for the intein-CBD fusion.

Common tag information:

  • Matrix: Chitin
  • Elution: DTT or B-ME
  • Yield/Purity: low milligram yields, 100% purity
  • # Steps: One step
  • Fusion Location: Internal
  • Affects Function?: Possibly, unlikely after intein cleavage

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]