Difference between revisions of "Part:BBa J70621"
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<partinfo>BBa_J70621 short</partinfo> | <partinfo>BBa_J70621 short</partinfo> | ||
| − | This is a RFC12 compatible version of <partinfo>BBa_T2000</partinfo>. It is a 7x his tag tail domain meant for C-terminal fusions. | + | This is a RFC12 compatible version of <partinfo>BBa_T2000</partinfo>. It is a 7x his tag tail domain meant for C-terminal fusions. . |
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 15:14, 9 August 2010
RFC12 7x His Tag Tail Domain
This is a RFC12 compatible version of BBa_T2000. It is a 7x his tag tail domain meant for C-terminal fusions. .
Usage and Biology
Polyhistidine tags are a very common purification tag used to separate or identify a target protein.
Common tag information:
- Matrix: Divalent Metal (Ni2+ is most common)
- Yield/Purity: 20%-80% pure, but depending on the methodology can be higher
- # Steps: One step
- Fusion Location: C-terminus (N-terminus is BBa_J70609)
- Affects Function?: Possible, but rare
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]