Difference between revisions of "Part:BBa J70620:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70620 short</partinfo>
 
<partinfo>BBa_J70620 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
Primers:
 
Primers:
−
 
+
  GTTTCTT C GAATTC GCGGCCGC T ACTAGT AAAATCGAAGAAGGTAAACTGGTAATCTGG, malE-F
−
 
+
  GTTTCTT C CTGCAG CGGCCGC T GCTAGC GTTGTTGTTATTGTTATTGTTGTTGTTGTTCGAG, malE-RN
 +
  GTTTCTT C CTGCAG CGGCCGC T GCTAGC AGTCTGCGCGTCTTTCAGGGC, malE-R
  
 
===Source===
 
===Source===
 +
NEB:[http://www.neb.com/nebecomm/products/productn8076.asp pMAL-c2x]
  
−
<a href="http://www.neb.com/nebecomm/products/productn8076.asp">pMAL-c2x</a>
 
  
 
===References===
 
===References===
 +
<biblio>
 +
#Kimple pmid=18429272
 +
#Terpe pmid=12536251
 +
</biblio>

Latest revision as of 15:09, 9 August 2010

RFC12 Maltose Binding Protein Internal Domain [malE]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 55


Design Notes

Primers: 

 GTTTCTT C GAATTC GCGGCCGC T ACTAGT AAAATCGAAGAAGGTAAACTGGTAATCTGG, malE-F
 GTTTCTT C CTGCAG CGGCCGC T GCTAGC GTTGTTGTTATTGTTATTGTTGTTGTTGTTCGAG, malE-RN
 GTTTCTT C CTGCAG CGGCCGC T GCTAGC AGTCTGCGCGTCTTTCAGGGC, malE-R

Source

NEB:[http://www.neb.com/nebecomm/products/productn8076.asp pMAL-c2x]


References

<biblio>

  1. Kimple pmid=18429272
  2. Terpe pmid=12536251

</biblio>