Difference between revisions of "Part:BBa K330000:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K330000===
 
===Applications of BBa_K330000===
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Protein tag
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===Characterization of BBa_K330000===
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To demonstrate that this biobirck, “FLAG-tag encoding sequence flanked by standard BioBrick™ prefix and suffix”, works as expected, a construct FLAGtag – EGFP was built and ligated into mammalian expression vector pUHD-P3. HtTA1 cells were transfected with this plasmid and observed under florescence microscope 48 hours after transfection. The cells were then lysed and protein extracts obtained. The extracted proteins were then subjected to SDS-PAGE and blotted with M2 (Sigma, anti-flagtag). HtTA1 cells transfected with empty pUHD-P3 were used as control.
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The results are as follows:
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<html>
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<div style="text-align:center"><img src="https://static.igem.org/mediawiki/parts/8/81/BBa_K330000.jpg" width="700px" border="2px"/>
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===User Reviews===
 
===User Reviews===

Latest revision as of 17:42, 23 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K330000

Protein tag


Characterization of BBa_K330000

To demonstrate that this biobirck, “FLAG-tag encoding sequence flanked by standard BioBrick™ prefix and suffix”, works as expected, a construct FLAGtag – EGFP was built and ligated into mammalian expression vector pUHD-P3. HtTA1 cells were transfected with this plasmid and observed under florescence microscope 48 hours after transfection. The cells were then lysed and protein extracts obtained. The extracted proteins were then subjected to SDS-PAGE and blotted with M2 (Sigma, anti-flagtag). HtTA1 cells transfected with empty pUHD-P3 were used as control.


The results are as follows:


User Reviews

UNIQ1bf32ecfbb989bdd-partinfo-00000001-QINU UNIQ1bf32ecfbb989bdd-partinfo-00000002-QINU