Difference between revisions of "Part:BBa K299000:Design"

 
(→‎Design Notes)
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K299000 short</partinfo>
 
<partinfo>BBa_K299000 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
J23100 used here is part of the Anderon RBS biobrick J61100.
 
J23100 used here is part of the Anderon RBS biobrick J61100.
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(Anderson RBSes comes in modified vectors)  
+
(Anderson RBSes come in modified psb1a2 vectors)
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+
  
 +
...Biobrick Prefix..................J23100.................XbaI....RBS Part.....Biobrick Suffix... 
 +
gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctagaGAAAGAGGGGACAAactagtagcggccgctgcag
  
 
===Source===
 
===Source===

Latest revision as of 00:14, 20 July 2010

RBS measurement device J23100 J61100 GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

J23100 used here is part of the Anderon RBS biobrick J61100. (Anderson RBSes come in modified psb1a2 vectors)

...Biobrick Prefix..................J23100.................XbaI....RBS Part.....Biobrick Suffix... gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctagaGAAAGAGGGGACAAactagtagcggccgctgcag

Source

registry

References