Difference between revisions of "Part:BBa P1014"

Latest revision as of 16:35, 2 July 2010

gentamicin resistance cassette aph(2)

Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155

Conveys resistance to genamicin at up to 12 ug/ml. It conveys low levels of resistance to kanamycin, at 1.5 ug/ml. E. coli NEB10B cells are sensitive to gentamicin at less than 0.3 ug/ml. Selection for this cassette carried out at 4 ug/ml will allow growth in Gentamicin, but inhibit other growth. With standard kanamycin concentrations of 35 ug/ml, this cassette will not convey Kanamycin resistance.

Gentamicin is an important antibiotic for use in human disease. Its use should be carefully considered and alternatives sought when used in a context promoting easy horizontal transfer of the cassette to potential human pathogens, for example in mobilizable plasmids or in transposable elements (insertions sequences).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 Novel gentamicin resistance gene.  Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray.  This, in turn was derived from Genbank AF016483.  See pmid=16391062, pmid=9593155
-Conveys resistance to Genamicin at up to 25 ug/ml.  It conveys low levels of resistance to Kanamycin, at 1.5 ug/ml. E. coli NEB10B cells are sensitive to Gentamicin at less than 0.3 ug/ml.  Selection for this cassette carried out at 5 ug/ml will allow growth in Gentamicin, but inhibit other growth.  With standard Kanamycin concentrations of 35 ug/ml, this cassette will not convey Kanamycin resistance.
+Conveys resistance to genamicin at up to 12 ug/ml.  It conveys low levels of resistance to kanamycin, at 1.5 ug/ml. E. coli NEB10B cells are sensitive to gentamicin at less than 0.3 ug/ml.  Selection for this cassette carried out at 4 ug/ml will allow growth in Gentamicin, but inhibit other growth.  With standard kanamycin concentrations of 35 ug/ml, this cassette will not convey Kanamycin resistance.
 Gentamicin is an important antibiotic for use in human disease.  Its use should be carefully considered and alternatives sought when used in a context promoting easy horizontal transfer of the cassette to potential human pathogens, for example in mobilizable plasmids or in transposable elements (insertions sequences).