Difference between revisions of "Part:BBa K416003:Design"

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===Design Notes===
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===Design Notes===  
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There was an illegal Xbal1 site at bp 101 and we removed it through altering the primers necessary for biobricking the sequence.
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Originally this part contained an illegal Xbal1 site at bp 101. Because it was near the 3' terminus of the biobrick, we were able to remove it using the reverse primer when biobricking the part.
  
 
===Source===
 
===Source===

Latest revision as of 00:24, 28 October 2010

Yeast Secretion Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Originally this part contained an illegal Xbal1 site at bp 101. Because it was near the 3' terminus of the biobrick, we were able to remove it using the reverse primer when biobricking the part.

Source

This part was sent to use from Dr. Sheldon Park of the University of Buffalo.

References

Gene. 1991 Oct 15;106(2):267-71. Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences. Clements JM, Catlin GH, Price MJ, Edwards RM.