Difference between revisions of "Part:BBa J70593"

Latest revision as of 18:32, 18 June 2010

RFC12 ATG Head Domain

A RFC12 compatible part that codes for a single amino acid, methionine. This is a head domain meant to be assembled directly in front of internal domains.

Please remember that modification of the first few codons can drastically affect translation efficiency. If you are worried about modification of the N-terminal, design your own head domain with the first two or three codons included.

This part will leave the RFC12 scar of GCTAGT [Ala-Ser] when assembled with a downstream protein. So the first three translated amino acids will be Met-Ala-Ser.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_J70593 short</partinfo>
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 A RFC12 compatible part that codes for a single amino acid, methionine.  This is a head domain meant to be assembled directly in front of internal domains.
-Please remember that modification of the first few codons can drastically affect translation efficiency. If you are worried about modification of the N-terminal design your own head domain with the first two or three codons included.
+Please remember that modification of the first few codons can drastically affect translation efficiency. If you are worried about modification of the N-terminal, design your own head domain with the first two or three codons included.
+This part will leave the RFC12 scar of GCTAGT [Ala-Ser] when assembled with a downstream protein. So the first three translated amino acids will be Met-Ala-Ser.
 <!-- Add more about the biology of this part here