Difference between revisions of "Part:BBa K398005:Design"
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<partinfo>BBa_K398005 short</partinfo> | <partinfo>BBa_K398005 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | Nucleotide sequence optimized for expression in E.coli. | + | Nucleotide sequence optimized for expression in E.coli. The restriction sites for XbaI, SpeI, EcoRI and PstI were removed. You can find the original sequence [http://www.ncbi.nlm.nih.gov/nuccore/AB040809.1 here] |
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===Source=== | ===Source=== | ||
− | Genbank | + | Genbank: [http://www.ncbi.nlm.nih.gov/nuccore/AB040809.1 AB040809.1] |
===References=== | ===References=== | ||
+ | Kato,T., ''et al.'' Gene Cloning of an alcohol dehydrogenase from thermophilic alkane-degrading Bacillus thermoleovorans B23, J. Biosci. Bioeng. 91 (1), 100-102 (2001). |
Latest revision as of 14:50, 26 October 2010
Medium-Chain Alcohol Dehydrogenase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Nucleotide sequence optimized for expression in E.coli. The restriction sites for XbaI, SpeI, EcoRI and PstI were removed. You can find the original sequence [http://www.ncbi.nlm.nih.gov/nuccore/AB040809.1 here]
Source
Genbank: [http://www.ncbi.nlm.nih.gov/nuccore/AB040809.1 AB040809.1]
References
Kato,T., et al. Gene Cloning of an alcohol dehydrogenase from thermophilic alkane-degrading Bacillus thermoleovorans B23, J. Biosci. Bioeng. 91 (1), 100-102 (2001).