Difference between revisions of "Part:BBa K416002"

 
 
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<partinfo>BBa_K416002 short</partinfo>
 
<partinfo>BBa_K416002 short</partinfo>
  
The antibody for the c-myc transcription factor. This protein binds to Enhancer Box sequences and recruits histone acetyltransferases, serving as a traditional transcription factor and a regulator of global chromatin structure.
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The loxP site is the substrate of the Cre recombinase. In nature the site is a palidromic 34bp sequence with a directional 8bp core. Because the site is not a multiple of 3, if it is used within coding regions it will throw the remainder of the sequence out of frame. We modified the loxP sequence by adding 2 bases to the 3' end. The resulting biobrick can now be used within transcribed assemblies without modifying the frame or the surrounding biobricks.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:03, 28 October 2010

36 Base Pair LoxP

The loxP site is the substrate of the Cre recombinase. In nature the site is a palidromic 34bp sequence with a directional 8bp core. Because the site is not a multiple of 3, if it is used within coding regions it will throw the remainder of the sequence out of frame. We modified the loxP sequence by adding 2 bases to the 3' end. The resulting biobrick can now be used within transcribed assemblies without modifying the frame or the surrounding biobricks.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]