Difference between revisions of "Help:Protocols/SOB"

(New page: == Summary == SOB Medium. Used in growing bacteria for preparing chemically competent cells == Ingredients == *0.5% (w/v) yeast extract *2% (w/v) tryptone *10 mM NaCl *2.5 mM KCl *20 mM ...)
 
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== Summary ==
 
== Summary ==
 
SOB Medium.  Used in growing bacteria for preparing chemically competent cells
 
SOB Medium.  Used in growing bacteria for preparing chemically competent cells

Latest revision as of 11:33, 11 June 2017

Summary

SOB Medium. Used in growing bacteria for preparing chemically competent cells

Ingredients

  • 0.5% (w/v) yeast extract
  • 2% (w/v) tryptone
  • 10 mM NaCl
  • 2.5 mM KCl
  • 20 mM MgSO4

Per liter:

  • 5 g yeast extract
  • 20 g tryptone
  • 0.584 g NaCl
  • 0.186 g KCl
  • 2.4 g MgSO4

Note: Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.

SOB medium is also available dry premixed from Difco, 0443-17.

Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.

15/10 medium

Growth of competent TOP10 cells in Example 2 of the Bloom05 patent is performed in 15/10 broth, which is similar to SOB:

  • 1.5% yeast extract
  • 1% Bacto-Tryptone
  • 10mM NaCl
  • 2mM KCl
  • 10 mM MgCl2
  • 10 mM MgSO4

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

SOB supposedly stands for super optimal broth.