Difference between revisions of "Part:BBa J70565"
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This part includes a fusion of the coding sequences for the luxA and luxB genes of the organism Xenorhabdus luminescens, along with luxA's native ribosomal binding site. | This part includes a fusion of the coding sequences for the luxA and luxB genes of the organism Xenorhabdus luminescens, along with luxA's native ribosomal binding site. | ||
| − | The coding regions provided by K216008,luciferase LuxAB of Xenorhabdus luminescens, were used in the fusion gene. Via a standard fusion PCR reaction luxA's stop codon was removed and the intervening nucleotides were replaced with a 10 amino Gly-Ser linker. In addition, the nonfunctional SpeI cut site in K216008 was fixed so that the part could be assembled with others in the future. | + | The coding regions provided by [https://parts.igem.org/wiki/index.php?title=Part:BBa_K216008 K216008],luciferase LuxAB of Xenorhabdus luminescens, were used in the fusion gene. Via a standard fusion PCR reaction luxA's stop codon was removed, luxB's start codon was removed, and the intervening nucleotides were replaced with a 10 amino Gly-Ser linker. In addition, the nonfunctional SpeI cut site in K216008 was fixed so that the part could be assembled with others in the future. |
LuxAB codes for a dimer that in the presence of decanal, produces light. This decanal can either be provided in-vitro with n-Decanal, or in-vivo by the luxCDABE cassete (which will produce tetradecanal). | LuxAB codes for a dimer that in the presence of decanal, produces light. This decanal can either be provided in-vitro with n-Decanal, or in-vivo by the luxCDABE cassete (which will produce tetradecanal). | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 19:27, 30 March 2010
luxAB Fusion gene, Xenorhabdus luminescens
This part includes a fusion of the coding sequences for the luxA and luxB genes of the organism Xenorhabdus luminescens, along with luxA's native ribosomal binding site.
The coding regions provided by K216008,luciferase LuxAB of Xenorhabdus luminescens, were used in the fusion gene. Via a standard fusion PCR reaction luxA's stop codon was removed, luxB's start codon was removed, and the intervening nucleotides were replaced with a 10 amino Gly-Ser linker. In addition, the nonfunctional SpeI cut site in K216008 was fixed so that the part could be assembled with others in the future.
LuxAB codes for a dimer that in the presence of decanal, produces light. This decanal can either be provided in-vitro with n-Decanal, or in-vivo by the luxCDABE cassete (which will produce tetradecanal).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 530
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1049