Difference between revisions of "Part:BBa M30109"
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<partinfo>BBa_M30109 short</partinfo>
 
<partinfo>BBa_M30109 short</partinfo>
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Pbad regulated phycobilins (ho1 and pcyA) followed by Ptet regulated light sensor (Cph8). This should allow repressed Pbad to give low levels of accessory proteins and derepressed Ptet to crank out lots of light sensor protein
 
Pbad regulated phycobilins (ho1 and pcyA) followed by Ptet regulated light sensor (Cph8). This should allow repressed Pbad to give low levels of accessory proteins and derepressed Ptet to crank out lots of light sensor protein
  
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===Usage and Biology===
 
===Usage and Biology===
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[http://www.pnas.org/cgi/doi/10.1073/pnas.191375198 Gambetta and Lagarias] suggest that induction of the phycobilins before the apophytochrome (Cph8) can significantly increase its yield, possibly because the bilin can cotranslationally covalently attach and stabilize nascent apophytochrome, preventing misfolding.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_M30109 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_M30109 SequenceAndFeatures</partinfo>
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_M30109 parameters</partinfo>
 
<partinfo>BBa_M30109 parameters</partinfo>
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Latest revision as of 19:44, 14 July 2006

Light responsive system, dual regulation

Pbad regulated phycobilins (ho1 and pcyA) followed by Ptet regulated light sensor (Cph8). This should allow repressed Pbad to give low levels of accessory proteins and derepressed Ptet to crank out lots of light sensor protein

Usage and Biology

[http://www.pnas.org/cgi/doi/10.1073/pnas.191375198 Gambetta and Lagarias] suggest that induction of the phycobilins before the apophytochrome (Cph8) can significantly increase its yield, possibly because the bilin can cotranslationally covalently attach and stabilize nascent apophytochrome, preventing misfolding.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1702
    Illegal BglII site found at 4190
    Illegal BamHI site found at 65
    Illegal XhoI site found at 2297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1255
  • 1000
    COMPATIBLE WITH RFC[1000]