Difference between revisions of "Part:BBa K5359001:Design"

 
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===Design Notes===
 
===Design Notes===
 
RBS sequence calculated based on entire protein coding sequence.
 
RBS sequence calculated based on entire protein coding sequence.
−
 
−
 
  
 
===Source===
 
===Source===
  
−
RBS sequence sourced from bioinformatic calculator.
+
RBS sequence sourced from bioinformatic calculator (1).
−
SYFP2 sequence sourced from Kremers et al.
+
SYFP2 sequence sourced from Kremers et al (2).
  
 
===References===
 
===References===
 +
1. Salis, H., Mirsky, E. & Voigt, C. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol 27, 946–950 (2009).https://doi.org/10.1038/nbt.1568
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 +
2. Kremers, G.-J., Goedhart, J., van Munster, E. B., & Gadella, T. W. J. (2006). Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry, 45(21), 6570–6580. doi:10.1021/bi0516273

Latest revision as of 13:48, 2 October 2024


SYFP2 RBS + SYFP2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS sequence calculated based on entire protein coding sequence.

Source

RBS sequence sourced from bioinformatic calculator (1). SYFP2 sequence sourced from Kremers et al (2).

References

1. Salis, H., Mirsky, E. & Voigt, C. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol 27, 946–950 (2009).https://doi.org/10.1038/nbt.1568

2. Kremers, G.-J., Goedhart, J., van Munster, E. B., & Gadella, T. W. J. (2006). Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry, 45(21), 6570–6580. doi:10.1021/bi0516273