Difference between revisions of "Part:BBa K5120018:Design"
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
  
−
-Some silent mutations were made to meet the part assembly standards
+
Some silent mutations were made to meet the part assembly standards, pEAQ-DEST1 was modified to include a suffix and prefix
−
-pEAQ-DEST1 was modified to include a suffix and prefix
+
−
 
+
  
 
===Source===
 
===Source===
  
−
The code for pEAQ-DEST1 was taken from ncbi database
+
The code for pEAQ-DEST1 was taken from NCBI database
−
The gene for the Pfr Promoter comes from <i>Arabidopsis thaliana</i>
+
The gene for the Pfr Promoter comes from <i>Arabidopsis thaliana</i> genome by going 2000 bp upstream of the Pfr gene
 
The gene for Barnase comes from Part:BBa_K1172904 from the iGEM team iGEM13_Bielefeld-Germany
 
The gene for Barnase comes from Part:BBa_K1172904 from the iGEM team iGEM13_Bielefeld-Germany
  
 
===References===
 
===References===
 
<li>iGEM Parts Registry. (n.d.). Part:BBa_K1172904. Retrieved from https://parts.igem.org/Part:BBa_K1172904</li>
 
<li>iGEM Parts Registry. (n.d.). Part:BBa_K1172904. Retrieved from https://parts.igem.org/Part:BBa_K1172904</li>

Latest revision as of 11:38, 2 October 2024


Pfr Promoter + Barnase Kill Switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 10226
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 10226
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5173
    Illegal NotI site found at 10232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 10226
    Illegal BglII site found at 644
    Illegal BglII site found at 1657
    Illegal BglII site found at 1913
    Illegal BglII site found at 1933
    Illegal BglII site found at 8791
    Illegal BglII site found at 9775
    Illegal BamHI site found at 1146
    Illegal XhoI site found at 714
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 10226
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 10226
    Illegal XbaI site found at 10241
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2397
    Illegal NgoMIV site found at 3353
    Illegal NgoMIV site found at 3636
    Illegal NgoMIV site found at 4168
    Illegal NgoMIV site found at 5045
    Illegal NgoMIV site found at 5169
    Illegal NgoMIV site found at 6196
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Some silent mutations were made to meet the part assembly standards, pEAQ-DEST1 was modified to include a suffix and prefix

Source

The code for pEAQ-DEST1 was taken from NCBI database The gene for the Pfr Promoter comes from Arabidopsis thaliana genome by going 2000 bp upstream of the Pfr gene The gene for Barnase comes from Part:BBa_K1172904 from the iGEM team iGEM13_Bielefeld-Germany

References

  • iGEM Parts Registry. (n.d.). Part:BBa_K1172904. Retrieved from https://parts.igem.org/Part:BBa_K1172904