Difference between revisions of "Part:BBa K249017:Design"

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===Design Notes===
 
===Design Notes===
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Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the C-terminal Arginine Tag  
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Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the N-terminal Arginine Tag
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This part was designed as an inversion device for YFP fluorescence. Upon addition of Arabinose the fluorescence of the protein was expected to drop significantly. This was observed.
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[[Image:Results fig 11.png]] [[Image:Results fig 21.png]]
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===Source===
 
===Source===

Latest revision as of 15:05, 31 October 2009

N-terminal YFP --> Tet inverter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the N-terminal Arginine Tag

Source

BioBrick Registry and synthesized Arganine Tag

References