Difference between revisions of "Part:BBa K5175042:Design"
 
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===References===
 
===References===
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[20] WITTGENS A, TISO T, ARNDT T T, et al. Growth independent rhamnolipid production from glucose using the non-pathogenic <i>Pseudomonas putida</i> KT2440 [J]. Microb Cell Fact, 2011, 10: 80.<br><br>
+
[1] WITTGENS A, TISO T, ARNDT T T, et al. Growth independent rhamnolipid production from glucose using the non-pathogenic <i>Pseudomonas putida</i> KT2440 [J]. Microb Cell Fact, 2011, 10: 80.<br><br>
−
[21] LIU H, CHEN Y, ZHANG Y, et al. Enhanced production of polyhydroxyalkanoates in <i>Pseudomonas putida</i> KT2440 by a combination of genome streamlining and promoter engineering [J]. International Journal of Biological Macromolecules, 2022, 209: 117-24.<br><br>
+
[2] LIU H, CHEN Y, ZHANG Y, et al. Enhanced production of polyhydroxyalkanoates in <i>Pseudomonas putida</i> KT2440 by a combination of genome streamlining and promoter engineering [J]. International Journal of Biological Macromolecules, 2022, 209: 117-24.<br><br>
−
[22] 尹进, 车雪梅, 陈国强. 聚羟基脂肪酸酯的研究进展 [J]. 生物工程学报, 32(6): 726-37.<br><br>
+
[3] 尹进, 车雪梅, 陈国强. 聚羟基脂肪酸酯的研究进展 [J]. 生物工程学报, 32(6): 726-37.<br><br>

Latest revision as of 08:11, 2 October 2024


T7 promoter-phaZ-T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 985
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 492
    Illegal NgoMIV site found at 693
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a composite component consisting of the T7 promoter, lac operator, T7 terminator, target genes phaZ.
PhaZ encoded by phaZ is a poly(3-hydroxyalkanoate) depolymerase.


Source

phaZ is from Pseudomonas putida.

References

[1] WITTGENS A, TISO T, ARNDT T T, et al. Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440 [J]. Microb Cell Fact, 2011, 10: 80.

[2] LIU H, CHEN Y, ZHANG Y, et al. Enhanced production of polyhydroxyalkanoates in Pseudomonas putida KT2440 by a combination of genome streamlining and promoter engineering [J]. International Journal of Biological Macromolecules, 2022, 209: 117-24.

[3] 尹进, 车雪梅, 陈国强. 聚羟基脂肪酸酯的研究进展 [J]. 生物工程学报, 32(6): 726-37.