Difference between revisions of "Part:BBa K5127005:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.
+
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in <i>E.coli</i>.
  
  
  
 
===Source===
 
===Source===
−
Genome of Pseudomonas putida.
+
Genome of <i>Pseudomonas putida</i>.
  
  

Latest revision as of 11:57, 2 October 2024


pIacR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.


Source

Genome of Pseudomonas putida.


References

d’Oelsnitz, S., Stofel, S. K., Love, J. D., & Ellington, A. D. (2024). Snowprint: a predictive tool for genetic biosensor discovery. Communications Biology, 7(1). https://doi.org/10.1038/s42003-024-05849-8

Liang, C., Xiong, D., Zhang, Y., Mu, S., & Tang, S.-Y. (2014). Development of a novel uric-acid-responsive regulatory system in Escherichia coli. Applied Microbiology and Biotechnology, 99(5), 2267–2275. https://doi.org/10.1007/s00253-014-6290-6

Wei, W., Liu, Y., Hou, Y., Cao, S., Chen, Z., Zhang, Y., Cai, X., Yan, Q., Li, Z., Yuan, Y., Wang, G., Zheng, X., & Hao, H. (2023). Psychological stress-induced microbial metabolite indole-3-acetate disrupts intestinal cell lineage commitment. Cell Metabolism. https://doi.org/10.1016/j.cmet.2023.12.026