Difference between revisions of "Part:BBa K5492203:Design"

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The source of the part is its amino acid sequence which was retrieved from Uniprot (https://www.uniprot.org/uniprotkb/Q498N2/entry#sequences), and the work of iGEM20_SEHS-China [https://parts.igem.org/Part:BBa_K3684002 BBa_K3684002] which provided the dna sequence we improved on.
 
The source of the part is its amino acid sequence which was retrieved from Uniprot (https://www.uniprot.org/uniprotkb/Q498N2/entry#sequences), and the work of iGEM20_SEHS-China [https://parts.igem.org/Part:BBa_K3684002 BBa_K3684002] which provided the dna sequence we improved on.
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The spacer used in the sequence is located on our page [https://parts.igem.org/Part:BBa_K5492100, BBa_K5492100]
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===References===
 
===References===

Latest revision as of 06:51, 2 October 2024


Optimised DAO - pETDUET-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 129
    Illegal XbaI site found at 47
    Illegal PstI site found at 190
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 129
    Illegal PstI site found at 190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 129
    Illegal BglII site found at 2392
    Illegal BamHI site found at 123
    Illegal BamHI site found at 2067
    Illegal BamHI site found at 2321
    Illegal XhoI site found at 937
    Illegal XhoI site found at 2441
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 129
    Illegal XbaI site found at 47
    Illegal PstI site found at 190
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 129
    Illegal XbaI site found at 47
    Illegal PstI site found at 190
    Illegal NgoMIV site found at 2411
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction sites adapted to pETDUET-1, with adjustions also added for proper framing of the gene.


Source

The source of the part is its amino acid sequence which was retrieved from Uniprot (https://www.uniprot.org/uniprotkb/Q498N2/entry#sequences), and the work of iGEM20_SEHS-China BBa_K3684002 which provided the dna sequence we improved on.


References