Difference between revisions of "Part:BBa K5322020:Design"
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===References=== | ===References=== | ||
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+ | 1.Combinatorial alanine-scanning Kim L Morrison and Gregory A Weiss.Department of Chemistry, University of California, Irvine,CA 92697-2025, USA. | ||
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+ | 2.Kim,D. , Noh,M.H. , Park,M. , Kim,I. , Ahn,H. , Ye,D. , Jung,G.Y. ,& Kim , S.(2022).Enzyme activity engineering based on sequence co-evolution analysis.Metabolic Engineering,74(),49-60.https://doi. org/10.1016/j.ymben.2022.09.001 |
Latest revision as of 03:28, 2 October 2024
SOD Pro7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 412
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The team obtains the SOD-1 sequence from BBa_K2215003.
Source
To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.
References
1.Combinatorial alanine-scanning Kim L Morrison and Gregory A Weiss.Department of Chemistry, University of California, Irvine,CA 92697-2025, USA.
2.Kim,D. , Noh,M.H. , Park,M. , Kim,I. , Ahn,H. , Ye,D. , Jung,G.Y. ,& Kim , S.(2022).Enzyme activity engineering based on sequence co-evolution analysis.Metabolic Engineering,74(),49-60.https://doi. org/10.1016/j.ymben.2022.09.001