Difference between revisions of "Part:BBa K5233006:Design"
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===Design Notes===
 
===Design Notes===
 
Wild E. coli has low ability to transfer TPA into its body, so we transformed E. coli with plasmids carrying tpiA, tpiB, and tphC to transport TPA.
 
Wild E. coli has low ability to transfer TPA into its body, so we transformed E. coli with plasmids carrying tpiA, tpiB, and tphC to transport TPA.
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===References===
 
===References===
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Dierkes RF, Wypych A, Pérez-García P, Danso D, Chow J, Streit WR. An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl Environ Microbiol. 2023 Jan 31;89(1):e0160322. doi: 10.1128/aem.01603-22. Epub 2022 Dec 12. PMID: 36507653; PMCID: PMC9888244.

Latest revision as of 02:46, 2 October 2024


tpiA, a kind of tripartite tricarboxylate transporter(TTT) family protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1519
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 437
    Illegal NgoMIV site found at 1473
    Illegal AgeI site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Wild E. coli has low ability to transfer TPA into its body, so we transformed E. coli with plasmids carrying tpiA, tpiB, and tphC to transport TPA. .


Source

It comes from Comamonas thiooxidans strain S23 genomic sequence, we obtained it from NCBI

References

Dierkes RF, Wypych A, Pérez-García P, Danso D, Chow J, Streit WR. An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl Environ Microbiol. 2023 Jan 31;89(1):e0160322. doi: 10.1128/aem.01603-22. Epub 2022 Dec 12. PMID: 36507653; PMCID: PMC9888244.