Difference between revisions of "Part:BBa K273019:Design"

(→‎Source)
(→‎Source)
 
Line 12: Line 12:
 
===Source===
 
===Source===
  
−
Synechocystis sp.PCC6803
+
<i>Synechocystis sp PCC6803</i>
  
 +
5' -> 3'
  
−
All directions 5' -> 3'
+
Forward primer: CGCgaattcgcggccgcttctagagTCATAGCGGTTGCCCAATC 71,4°C annealing
  
−
Forward primer:
+
Reverse primer: GCGctgcagcggccgctactagtaACTTCTTGGCGATTGTATCTATAGGG 69,9°C annealing
−
CGCgaattcgcggccgcttctagagTCATAGCGGTTGCCCAATC 71,4°C annealing
+
−
 
+
−
Reverse primer:
+
−
GCGctgcagcggccgctactagtaACTTCTTGGCGATTGTATCTATAGGG 69,9°C annealing
+
  
 
===References===
 
===References===
  
 
[http://www.ncbi.nlm.nih.gov/pubmed/2129338 Copper-induced expression, cloning, and regulatory studies of the plastocyanin gene from the cyanobacterium Synechocystis sp. PCC 6803. <i>Plant Mol Biol. 1990 Oct;15(4):633-42</i>]
 
[http://www.ncbi.nlm.nih.gov/pubmed/2129338 Copper-induced expression, cloning, and regulatory studies of the plastocyanin gene from the cyanobacterium Synechocystis sp. PCC 6803. <i>Plant Mol Biol. 1990 Oct;15(4):633-42</i>]

Latest revision as of 12:10, 22 October 2009

PpetE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Synechocystis sp PCC6803

5' -> 3'

Forward primer: CGCgaattcgcggccgcttctagagTCATAGCGGTTGCCCAATC 71,4°C annealing

Reverse primer: GCGctgcagcggccgctactagtaACTTCTTGGCGATTGTATCTATAGGG 69,9°C annealing

References

[http://www.ncbi.nlm.nih.gov/pubmed/2129338 Copper-induced expression, cloning, and regulatory studies of the plastocyanin gene from the cyanobacterium Synechocystis sp. PCC 6803. Plant Mol Biol. 1990 Oct;15(4):633-42]