Difference between revisions of "Part:BBa K197030:Design"

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__NOTOC__
 
__NOTOC__
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<partinfo>GFP-LVA</partinfo>
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<partinfo>GFP-LVA short</partinfo>
  
 
<partinfo>BBa_K197030 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K197030 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
 +
This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at: <br>
 +
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]
  
 +
This part was made using a LR Gateway Transfer reaction. More information on this process can be found here:<br>
 +
[http://2008.igem.org/Team:UC_Berkeley/GatewayOverview LR Gateway Transfer Overview]
  
−
 
+
The donor plasmid pBca1256-Bjh1857, which contains the gene GFP-LVA, underwent a Gateway Transfer reaction with the recipient plasmid pBca1254AK.
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+
  
 
===Source===
 
===Source===
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synthesis
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Plasmid pBca1256-Bjh1857
  
 
===References===
 
===References===
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Miller WG, Leveau JH, Lindow SE. Improved gfp and inaZ broad-host-range promoter-probe vectors. Mol Plant Microbe Interact. 2000 Nov;13(11):1243-50. PMID: 11059491
+
Andersen JB et al (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. ''Appl. Environ. Microbiol.'' 64: 2240-2246.<br>
 +
Miller WG et al (2000). Improved gfp and inaZ broad-host-range promoter-probe vectors. ''Mol Plant Microbe Interact.'' 13(11): 1243-1250.

Latest revision as of 04:37, 22 October 2009

No part name specified with partinfo tag.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 716
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 716
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 716
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641


Design Notes

This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]

This part was made using a LR Gateway Transfer reaction. More information on this process can be found here:
[http://2008.igem.org/Team:UC_Berkeley/GatewayOverview LR Gateway Transfer Overview]

The donor plasmid pBca1256-Bjh1857, which contains the gene GFP-LVA, underwent a Gateway Transfer reaction with the recipient plasmid pBca1254AK.

Source

Plasmid pBca1256-Bjh1857

References

Andersen JB et al (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64: 2240-2246.
Miller WG et al (2000). Improved gfp and inaZ broad-host-range promoter-probe vectors. Mol Plant Microbe Interact. 13(11): 1243-1250.