Difference between revisions of "Part:BBa K5286003"
Maravalverde (Talk | contribs) (Re added the Main page content) |
Maravalverde (Talk | contribs) |
||
| Line 1: | Line 1: | ||
| − | This part contains Hypsibius dujardini's heat soluble protein | + | This part contains Hypsibius dujardini's heat soluble protein CAHS107838 coding sequence, and a 10XHisTag with a single stop codon, which under the expression of an inducible promoter allows for protein purification according to the protocol by Boothby et al.(2017): |
"Cells were then lysed by heat shock at 95°C for 15 min. Lysates were cooled at room temperature for 30 min. Insoluble components were removed by centrifugation at 20,000g and 10°C for 30 min." | "Cells were then lysed by heat shock at 95°C for 15 min. Lysates were cooled at room temperature for 30 min. Insoluble components were removed by centrifugation at 20,000g and 10°C for 30 min." | ||
The protein can then be further purified with a HisTag purification kit. | The protein can then be further purified with a HisTag purification kit. | ||
Latest revision as of 21:51, 1 October 2024
This part contains Hypsibius dujardini's heat soluble protein CAHS107838 coding sequence, and a 10XHisTag with a single stop codon, which under the expression of an inducible promoter allows for protein purification according to the protocol by Boothby et al.(2017):
"Cells were then lysed by heat shock at 95°C for 15 min. Lysates were cooled at room temperature for 30 min. Insoluble components were removed by centrifugation at 20,000g and 10°C for 30 min."
The protein can then be further purified with a HisTag purification kit.