Difference between revisions of "Part:BBa K197026:Design"

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===Design Notes===
 
===Design Notes===
−
This is a part of a repeat in the ice nucleation protien
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This part is in BglBricks Standard.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BglBricks Standard is available at:
−
 
+
  
 +
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
  
 
===Source===
 
===Source===
 +
<pre>
 
Construction of Ice Nucleation Repeats (INP repeats) Basic Part
 
Construction of Ice Nucleation Repeats (INP repeats) Basic Part
 
PCR Oeh001F/Oeh002R on pBca1256-Bca1346 (2296bp, EcoRI/BamHI)
 
PCR Oeh001F/Oeh002R on pBca1256-Bca1346 (2296bp, EcoRI/BamHI)
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eh001R Reverse BamHI oligo for <INP repeats>
 
eh001R Reverse BamHI oligo for <INP repeats>
 
gtcagGGATCCactgtcctgcccggccgttg
 
gtcagGGATCCactgtcctgcccggccgttg
 +
</pre>
  
 
===References===
 
===References===
 
Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340
 
Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340

Latest revision as of 05:22, 22 October 2009

INP-Repeats


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

Construction of Ice Nucleation Repeats (INP repeats) Basic Part
PCR Oeh001F/Oeh002R on pBca1256-Bca1346		(2296bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5			(EcoRI/BamHI, L)
Product is pBca9495AK-M10048      {<INP repeats>}
---
eh001F Forward EcoRI oligo for <INP repeats>
gtcagGAATTCatgAGATCTaaccacagtgatctaattgc
eh001R Reverse BamHI oligo for <INP repeats>
gtcagGGATCCactgtcctgcccggccgttg

References

Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340