Difference between revisions of "Part:BBa K5175036:Design"
 
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===Design Notes===
 
===Design Notes===
−
Nothing.
+
It is a composite component consisting of the T7 promoter, T7 terminator, target genes tphB, tpaK. It is responsible for oxidising the diol moiety (two hydroxyl groups) of DCD to a keto group to result in the production of PCA and transporting TPA.
 +
TphB is a dehydrogenase that oxidises the diol moiety (two hydroxyl groups) of DCD to a keto group, resulting in the production of PCA.
 +
TpaK is the TPA transporter protein.
  
  
  
 
===Source===
 
===Source===
 +
tphB is from <i>Comamonas sp.</i>E6.
 +
tpaK is from <i>Rhodococcus erythropolis</i>.
  
−
<i>Rhodococcus erythropolis</i>
 
  
 
===References===
 
===References===
 +
[1] SASOH M, MASAI E, ISHIBASHI S, et al. Characterization of the Terephthalate Degradation Genes of <i>Comamonas sp.</i> Strain E6 [J]. Applied and Environmental Microbiology, 2006, 72(3): 1825-32.<br><br>
 +
[2] KINCANNON W M, ZAHN M, CLARE R, et al. Biochemical and structural characterization of an aromatic ring-hydroxylating dioxygenase for terephthalic acid catabolism [J]. Proc Natl Acad Sci U S A, 2022, 119(13): e2121426119.<br><br>
 +
[3] HOSAKA M, KAMIMURA N, TORIBAMI S, et al. Novel tripartite aromatic acid transporter essential for terephthalate uptake in <i>Comamonas sp.</i> strain E6 [J]. Appl Environ Microbiol, 2013, 79(19): 6148-55.<br><br>

Latest revision as of 09:12, 2 October 2024


T7 promoter - tphB-tpaK-T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1906
    Illegal PstI site found at 2195
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2462
    Illegal PstI site found at 1906
    Illegal PstI site found at 2195
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 242
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1906
    Illegal PstI site found at 2195
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1906
    Illegal PstI site found at 2195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 964
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a composite component consisting of the T7 promoter, T7 terminator, target genes tphB, tpaK. It is responsible for oxidising the diol moiety (two hydroxyl groups) of DCD to a keto group to result in the production of PCA and transporting TPA. TphB is a dehydrogenase that oxidises the diol moiety (two hydroxyl groups) of DCD to a keto group, resulting in the production of PCA. TpaK is the TPA transporter protein.


Source

tphB is from Comamonas sp.E6. tpaK is from Rhodococcus erythropolis.


References

[1] SASOH M, MASAI E, ISHIBASHI S, et al. Characterization of the Terephthalate Degradation Genes of Comamonas sp. Strain E6 [J]. Applied and Environmental Microbiology, 2006, 72(3): 1825-32.

[2] KINCANNON W M, ZAHN M, CLARE R, et al. Biochemical and structural characterization of an aromatic ring-hydroxylating dioxygenase for terephthalic acid catabolism [J]. Proc Natl Acad Sci U S A, 2022, 119(13): e2121426119.

[3] HOSAKA M, KAMIMURA N, TORIBAMI S, et al. Novel tripartite aromatic acid transporter essential for terephthalate uptake in Comamonas sp. strain E6 [J]. Appl Environ Microbiol, 2013, 79(19): 6148-55.