Difference between revisions of "Part:BBa K5127003:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.
+
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in <i>E. coli</i>.
  
  
  
 
===Source===
 
===Source===
−
Genome of Vibrio cholerae.
+
Genome of <i>Vibrio cholerae</i>.
  
  

Latest revision as of 10:18, 2 October 2024


pBreR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 23
    Illegal NheI site found at 46
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E. coli.


Source

Genome of Vibrio cholerae.


References

Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., Bodenstein, S. W., Evans, D. A., Hung, C. C., O'Neill, M., Reiman, D., Tunyasuvunakool, K., Wu, Z., Žemgulytė, A., Arvaniti, E., ... Jumper, J. M. (2024). Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature, 630(493-500). https://doi.org/10.1038/s41586-024-07487-w

Beabout, K., Ehrenworth Breedon, A. M., Blum, S. M., Miklos, A. E., Lux, M. W., Chávez, J. L., & Goodson, M. S. (2022). Detection of Bile Acids in Complex Matrices Using a Transcription Factor-Based Biosensor. ACS Biomaterials Science & Engineering, 9(9), 5151–5162. https://doi.org/10.1021/acsbiomaterials.2c01006