Difference between revisions of "Part:BBa K5499006"

Latest revision as of 21:58, 1 October 2024

Trans-activator of transcription (TAT)

Trans-activator of transcription (TAT) peptide is an efficient cell-penetrating peptide, which could penetrate the plasma membrane as well as the nuclear envelop of majority of living cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Profile

Name: Trans-activator of Transcription (TAT)

Base Pairs: 33 bp

Origins: TAT is derived from the Human Immunodeficiency Virus (HIV).

Properties: TAT peptide can effectively cross the lipid bilayer of the plasma membrane, enabling intracellular delivery of cargo such as proteins, peptides, and nucleic acids.

TAT utilizes a receptor/transporter-independent pathway for internalization, allowing it to bypass traditional uptake mechanisms, which may be saturated in high concentrations.

Usage and Biology

The TAT protein is widely used in synthetic biology and gene therapy as a transduction agent. Its ability to penetrate cellular membranes allows researchers to deliver various therapeutic molecules, including proteins, peptides, and nucleic acids, directly into target cells. This capability is particularly useful for enhancing the efficiency of gene delivery systems and developing treatments for diseases such as cancer and genetic disorders. Its ability to cross the blood-brain barrier (BBB) makes it invaluable for targeting brain tissues.

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 <partinfo>BBa_K5499006 short</partinfo>
-Trans-activator of transcription (TAT) peptide is an efficient cell-penetrating peptide, which could penetrate the plasma membrane as well as the nuclear envelop of majority of living cells.Moreover, TAT can easily penetrate biofilm barriers of BBB and penetrate dense tumors tissues by triggering internalization through an unsaturated and receptor/transporter-independent pathway.
+Trans-activator of transcription (TAT) peptide is an efficient cell-penetrating peptide, which could penetrate the plasma membrane as well as the nuclear envelop of majority of living cells.
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 <!-- Add more about the biology of this part here-->
 ===Profile===
-Name: Trans-activator of Transcription (TAT) <br>
+Name: Trans-activator of Transcription (TAT)
-Base Pairs: 33 bp <br>
-Origins: TAT is derived from the Human Immunodeficiency Virus (HIV). It is a key regulatory protein encoded by the tat gene, which plays a crucial role in the viral replication process by enhancing the transcription of viral genes. <br>
-Properties: TAT functions as a transcriptional activator that enhances the expression of genes by recruiting host cellular transcription factors and modifying the transcriptional machinery. It is characterized by its ability to translocate across the cell membrane and facilitate the delivery of therapeutic proteins or nucleic acids into cells. This unique property makes TAT a valuable tool in gene therapy and drug delivery applications.
+Base Pairs: 33 bp
+Origins: TAT is derived from the Human Immunodeficiency Virus (HIV).
+Properties: TAT peptide can effectively cross the lipid bilayer of the plasma membrane, enabling intracellular delivery of cargo such as proteins, peptides, and nucleic acids.
+TAT utilizes a receptor/transporter-independent pathway for internalization, allowing it to bypass traditional uptake mechanisms, which may be saturated in high concentrations.
 ===Usage and Biology===
-The TAT protein is widely used in synthetic biology and gene therapy as a transduction agent. Its ability to penetrate cellular membranes allows researchers to deliver various therapeutic molecules, including proteins, peptides, and nucleic acids, directly into target cells. This capability is particularly useful for enhancing the efficiency of gene delivery systems and developing treatments for diseases such as cancer and genetic disorders. <br>
+The TAT protein is widely used in synthetic biology and gene therapy as a transduction agent. Its ability to penetrate cellular membranes allows researchers to deliver various therapeutic molecules, including proteins, peptides, and nucleic acids, directly into target cells. This capability is particularly useful for enhancing the efficiency of gene delivery systems and developing treatments for diseases such as cancer and genetic disorders. Its ability to cross the blood-brain barrier (BBB) makes it invaluable for targeting brain tissues.
-TAT plays a crucial role in the life cycle of HIV by enhancing the transcription of viral genes, allowing for efficient replication of the virus. It binds to the TAR (transactivation response) element present in the viral RNA, recruiting host transcription factors and facilitating the elongation of RNA polymerase II during transcription. Additionally, TAT is involved in various cellular processes, including apoptosis and cell signaling, which further underscores its importance in both viral biology and potential therapeutic applications.