Difference between revisions of "Part:BBa K257013:Experience"
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x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar without glucose neither arabinose | x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar without glucose neither arabinose | ||
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[[Image:PbadLacIYFP fluo.jpg|360px]] | [[Image:PbadLacIYFP fluo.jpg|360px]] | ||
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x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction on LB agar without glucose neither arabinose | x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction on LB agar without glucose neither arabinose | ||
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[[Image:PbadLacIYFP glucose trans1.jpg|360px]] | [[Image:PbadLacIYFP glucose trans1.jpg|360px]] | ||
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| − | x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar with glucose | + | x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar with 1% glucose |
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[[Image:PbadLacIYFP glucose fluo1.jpg|360px]] | [[Image:PbadLacIYFP glucose fluo1.jpg|360px]] | ||
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| − | x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction plated on LB agar with glucose | + | x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction plated on LB agar with 1% glucose |
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| − | + | '''Conclusion :''' | |
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| − | + | In presence of glucose pBad is repressed and then no fluorescence is normally observed. Accordingly bacteria with our construction are less fluorescent in presence of glucose than in the control. | |
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Latest revision as of 04:00, 22 October 2009
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Applications of BBa_K257013
x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar without glucose neither arabinose
x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction on LB agar without glucose neither arabinose
x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar with 1% glucose
x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction plated on LB agar with 1% glucose
Conclusion :
In presence of glucose pBad is repressed and then no fluorescence is normally observed. Accordingly bacteria with our construction are less fluorescent in presence of glucose than in the control.