Difference between revisions of "Part:BBa K257013:Experience"

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[[Image:PbadLacIYFP trans-1.jpg|360px]]
 
[[Image:PbadLacIYFP trans-1.jpg|360px]]
 
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x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar without glucose neither arabinose
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[[Image:PbadLacIYFP fluo.jpg|360px]]
 
[[Image:PbadLacIYFP fluo.jpg|360px]]
 
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x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction on LB agar without glucose neither arabinose
  
  
 
[[Image:PbadLacIYFP glucose trans1.jpg|360px]]
 
[[Image:PbadLacIYFP glucose trans1.jpg|360px]]
 
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x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar with 1% glucose
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[[Image:PbadLacIYFP glucose fluo1.jpg|360px]]
 
[[Image:PbadLacIYFP glucose fluo1.jpg|360px]]
 
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x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction plated on LB agar with 1% glucose
  
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===User Reviews===
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'''Conclusion :'''
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{|width='80%' style='border:1px solid gray'
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In presence of glucose pBad is repressed and then no fluorescence is normally observed. Accordingly bacteria with our construction are less fluorescent in presence of glucose than in the control.
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<partinfo>BBa_K257013 AddReview number</partinfo>
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<I>Username</I>
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|width='60%' valign='top'|
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Enter the review inofrmation here.
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|};
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<!-- DON'T DELETE --><partinfo>BBa_K257013 EndReviews</partinfo>
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Latest revision as of 04:00, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K257013


PbadLacIYFP trans-1.jpg
x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar without glucose neither arabinose


PbadLacIYFP fluo.jpg
x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction on LB agar without glucose neither arabinose


PbadLacIYFP glucose trans1.jpg
x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar with 1% glucose


PbadLacIYFP glucose fluo1.jpg
x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction plated on LB agar with 1% glucose


Conclusion :
In presence of glucose pBad is repressed and then no fluorescence is normally observed. Accordingly bacteria with our construction are less fluorescent in presence of glucose than in the control.