Difference between revisions of "Part:BBa K5492010"

 
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===Experiments===
 
===Experiments===
==PCR==
+
==PCR - optimised DAO==
 
We have found the following PCR protocol the most optimal for the part:
 
We have found the following PCR protocol the most optimal for the part:
 
https://static.igem.wiki/teams/5492/registry/dao-pcr-plan.png
 
https://static.igem.wiki/teams/5492/registry/dao-pcr-plan.png
  
Optimised dao:
 
  
 
We added the following substrates in order:
 
We added the following substrates in order:
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+
We added 6X DNA Loading dye to each PCR tubes.
 
+
We added the 6X DNA Loading dye to each PCR tubes.
+
 
We loaded the wells of the gels with 12μL of DAO solution in the following order:  
 
We loaded the wells of the gels with 12μL of DAO solution in the following order:  
1. D31O
 
2. D32O
 
3. D33O
 
4. D34O
 
5. Ladder
 
  
  
  
 
https://static.igem.wiki/teams/5492/registry/opt-dao-pcr.png
 
https://static.igem.wiki/teams/5492/registry/opt-dao-pcr.png
 +
 +
1.D31O 2.D32O 3.D33O 4.D34O 5.Ladder
  
 
<partinfo>BBa_K5492010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5492010 SequenceAndFeatures</partinfo>

Latest revision as of 14:58, 1 October 2024


Twist adapter forward primer

Forward primer sequence of the adapter sequence utilised by Twist Bioscience. Utilised in PCR reactions for part BBa_K5492202 and BBa_K5492203.

Experiments

PCR - optimised DAO

We have found the following PCR protocol the most optimal for the part: dao-pcr-plan.png


We added the following substrates in order:

dao-pcr-substr.png


We added 6X DNA Loading dye to each PCR tubes. We loaded the wells of the gels with 12μL of DAO solution in the following order:


opt-dao-pcr.png

1.D31O 2.D32O 3.D33O 4.D34O 5.Ladder


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]