Difference between revisions of "Part:BBa K5323893:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
In order to enable the normal expression of this gene in <i>Acidithiobacillus ferrooxidans</i>, we performed codon optimization.
+
A component that determines whether <i>mazF</i> gene is expressed or not by a gold detector.
  
  
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===Source===
 
===Source===
  
−
A composite part assembled by RBS(BBa_J34801), <i>golS</i> (BBa_K4468005), <i>P<sub>gol</sub></i>(BBa_K4468001), <i>cI</i> (BBa_R1051), <i>P<sub>tac</sub></i>(BBa_K180000), TT(BBa_B0015), <i>mazF</i>(BBa_K1096002) and <i>PR</i>(BBa_K2229450).
+
A composite part assembled by RBS(BBa_J34801), <i>golS</i> (BBa_K4468005), <i>P<sub>gol</sub></i>(BBa_K4468001), <i>cI</i> (BBa_R1051), <i>P<sub>tac</sub></i>(BBa_K180000), TT(BBa_B0015), <i>mazF</i>(BBa_K1096002) and <i>P<sub>R</sub></i>(BBa_K2229450).
  
 
===References===
 
===References===

Latest revision as of 15:08, 1 October 2024


Ptac-RBS-golS-Pgol-RBS-cI-TT-PR-RBS-mazF-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1635
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3721
    Illegal AgeI site found at 1969
    Illegal AgeI site found at 2318
    Illegal AgeI site found at 3256
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A component that determines whether mazF gene is expressed or not by a gold detector.


Source

A composite part assembled by RBS(BBa_J34801), golS (BBa_K4468005), Pgol(BBa_K4468001), cI (BBa_R1051), Ptac(BBa_K180000), TT(BBa_B0015), mazF(BBa_K1096002) and PR(BBa_K2229450).

References