Difference between revisions of "Part:BBa K5322013"

(→‎Construction of the plasmid)
 
(One intermediate revision by one other user not shown)
Line 24: Line 24:
 
</style>
 
</style>
 
<div class="center-img">
 
<div class="center-img">
−
<img src="https://static.igem.wiki/teams/5322/wet-lab/soxs-opju.png" alt="pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7" width="300">
+
<img src="https://static.igem.wiki/teams/5322/wet-lab/soxs-opju.png" alt="pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7" width="600">
−
<p align="center"><b>Figure 1-1</b> gene circuit pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7</p>
+
<p align="center"><b>Figure 2-1</b> gene circuit pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7</p>
 
</div>
 
</div>
  
Line 43: Line 43:
 
</style>
 
</style>
 
<div class="center-img">
 
<div class="center-img">
−
<img src="https://static.igem.wiki/teams/5322/t-imgs-2/15-p-soxr-t-psoxs-rbs-sod-t.png" alt="pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7" width="300">
+
<img src="https://static.igem.wiki/teams/5322/wet-lab/p-soxr-t-psoxs-rbs-sod-t-map.png" alt="pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7" width="600">
−
<p align="center"><b>Figure 2-1</b> Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7</p>
+
<p align="center"><b>Figure 3-1</b> Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7</p>
 
</div>
 
</div>
  

Latest revision as of 11:53, 2 October 2024

pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.

Usage and Biology

Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7,the pET29a vector is used for high-level expression in E. coli. The expression of SOD-1 is controlled by the NO-responsive promoter SoxR/SoxS. The ribosome binding site (RBS) ensures efficient translation of mRNA, while the T7 terminator provides a clean and efficient end for transcription. This system is designed to express SOD only in the intestinal inflammation site and exert its antioxidant effect.

Structural design

Our team designed the gene circuit shown in the figure below: pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.

pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7

Figure 2-1 gene circuit pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7

Construction of the plasmid

Our team designed the plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7, and the plasmid map is illustrated below.

pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7

Figure 3-1 Plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 552
    Illegal NheI site found at 1157
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]