Difference between revisions of "Part:BBa K243002"
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<partinfo>BBa_K243002 short</partinfo><br> | <partinfo>BBa_K243002 short</partinfo><br> | ||
| − | DsbA | + | The signal sequence of the DsbA protein linked to the coding sequence of another protein enables export of the latter into the periplasm. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The DsbA gene encodes a periplasmic disulfide bond isomerase which posses a signal sequence. The protein is localized in the periplasm and catalyzes the formation of disulfide bonds. It therefore enables the correct folding of proteins in this compartment.<br> | ||
The signal sequence of the DsbA protein linked to the coding sequence of another protein enables export of the latter into the periplasm. The accumulation of the expressed protein in the periplasm can be used for the purification of the protein and to prevent interaction with cytoplasmic components. | The signal sequence of the DsbA protein linked to the coding sequence of another protein enables export of the latter into the periplasm. The accumulation of the expressed protein in the periplasm can be used for the purification of the protein and to prevent interaction with cytoplasmic components. | ||
[[Image:Freiburg 09 DsbA grafik.jpg|550x550px]]<br> | [[Image:Freiburg 09 DsbA grafik.jpg|550x550px]]<br> | ||
Latest revision as of 02:53, 22 October 2009
DsbA signal sequence (enables periplasm export)
The signal sequence of the DsbA protein linked to the coding sequence of another protein enables export of the latter into the periplasm.
Usage and Biology
The DsbA gene encodes a periplasmic disulfide bond isomerase which posses a signal sequence. The protein is localized in the periplasm and catalyzes the formation of disulfide bonds. It therefore enables the correct folding of proteins in this compartment.
The signal sequence of the DsbA protein linked to the coding sequence of another protein enables export of the latter into the periplasm. The accumulation of the expressed protein in the periplasm can be used for the purification of the protein and to prevent interaction with cytoplasmic components.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]