Difference between revisions of "Part:BBa K239001:Experience"

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The device responded to various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%). Possibly it can also be used to detect presence of heterogeneous unfolded proteins in the periplasmic space (e.g. target pharmaceuticals) or to detect high levels of shear stress during cultivation in a bioreactor.
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The promoter respondeds to various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%), which can posibbly cause protein mis-folding in baterial cell(Detailed mechanism is unknown yet). Possibly it can also be used to detect presence of heterogeneous unfolded proteins in the periplasmic space (e.g. target pharmaceuticals) or to detect high levels of shear stress during cultivation in a bioreactor.
  
 
Compare to the related DegP promoter (BBa_K239000), Spy promoter, which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Most importantly, spy promoter showed no respond to stationary phase.  
 
Compare to the related DegP promoter (BBa_K239000), Spy promoter, which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Most importantly, spy promoter showed no respond to stationary phase.  
  
 
For detailed information please visit [http://2009.igem.org/Team:UCL_London/From_the_lab/Results#Growth_Cruves_Experiments_II UCL_London iGEM Team Wiki Growth_Cruves_Experiments_II]
 
For detailed information please visit [http://2009.igem.org/Team:UCL_London/From_the_lab/Results#Growth_Cruves_Experiments_II UCL_London iGEM Team Wiki Growth_Cruves_Experiments_II]

Latest revision as of 02:11, 22 October 2009

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Please enter how you used this part and how it worked out.

Applications of BBa_K239001

User Reviews

UNIQ3927db7d4f8a4a68-partinfo-00000000-QINU UNIQ3927db7d4f8a4a68-partinfo-00000001-QINU

The promoter respondeds to various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%), which can posibbly cause protein mis-folding in baterial cell(Detailed mechanism is unknown yet). Possibly it can also be used to detect presence of heterogeneous unfolded proteins in the periplasmic space (e.g. target pharmaceuticals) or to detect high levels of shear stress during cultivation in a bioreactor.

Compare to the related DegP promoter (BBa_K239000), Spy promoter, which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Most importantly, spy promoter showed no respond to stationary phase.

For detailed information please visit [http://2009.igem.org/Team:UCL_London/From_the_lab/Results#Growth_Cruves_Experiments_II UCL_London iGEM Team Wiki Growth_Cruves_Experiments_II]