Difference between revisions of "Part:BBa K5096070"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K5096070 short</partinfo> | <partinfo>BBa_K5096070 short</partinfo> | ||
| + | This sequence codes for the guide sequence of the sgRNA70. This sequence is derived from the inhA gene of Mycobacterium tuberculosis. Among M. tuberculosis genes, the inhA gene was selected due to its low mutation rate and its critical role in M. tuberculosis’s pathogenicity and survival (Marrakchi et al., 2014). By inhibiting inhA with our CRISPRi system, the sgRNA70 aims to disrupt M. tuberculosis's pathogenicity. | ||
| + | This part is critical for locating the non-coding strand of the target DNA sequence, since it has complementary base pairs of inhA target. | ||
Latest revision as of 00:16, 2 October 2024
sg70 This sequence codes for the guide sequence of the sgRNA70. This sequence is derived from the inhA gene of Mycobacterium tuberculosis. Among M. tuberculosis genes, the inhA gene was selected due to its low mutation rate and its critical role in M. tuberculosis’s pathogenicity and survival (Marrakchi et al., 2014). By inhibiting inhA with our CRISPRi system, the sgRNA70 aims to disrupt M. tuberculosis's pathogenicity. This part is critical for locating the non-coding strand of the target DNA sequence, since it has complementary base pairs of inhA target.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]