Difference between revisions of "Part:BBa K218020:Design"
 
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The plasmid containing LuxOD47A (pJAF544) was obtained from Bassler lab (Freeman and Bassler, 1999).
 
The plasmid containing LuxOD47A (pJAF544) was obtained from Bassler lab (Freeman and Bassler, 1999).
  
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>gi|156972381:c2983472-2982069 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence Nucleotide number 141 has been mutated from T to G, thus bases 139-141 have been changed from GAT (Aspartic acid, D) to GAG (Glutamine, E).
 
  
  

Latest revision as of 03:34, 22 October 2009

LuxO D47A followed by terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 824
    Illegal SapI.rc site found at 786


Design Notes

The plasmid containing LuxOD47A (pJAF544) was obtained from Bassler lab (Freeman and Bassler, 1999).



Source

Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.


References